Device
Ateam

Part:BBa_K1023016

Designed by: Shreyans Chordia   Group: iGEM13_Wageningen_UR   (2013-09-27)


FRET based ATP biosensor for expression in Aspergillus niger

Consists of cfp-yfp fret pair that responds to changes in ATP concentration

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1082
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1345


[edit]
Categories
//chassis/multihost
//function/fret
//function/sensor
Parameters
chassisAspergillus niger, Escherichia coli, Saccharomyces cerevisiae,Helacyton gartleri
emissionCFP - 475 nm , YFP - 527 nm
excitationCFP - 435 nm , YFP - 515 nm
functionDetect of in vivo ATP levels in Aspergillus niger
typeFRET based (donor-CFP, acceptor-YFP)